Response of Leaves and Stem Leek (Allium porrum L.) Cultivars to Salt Stress on Growth, Yield, Mineral Composition, Organic and Antioxidant Compounds Cultivated in the Far North Region of Cameroon
Main Article Content
Abstract
Context: In the world, millions of hectares of cultivated land are affected by salt, making salinity a major constraint for plant production.
Objective: This investigation was conducted to examine the influence of salinity on plant growth, yield, and chemical compounds of leaves and stems of leek cultivars. Experiments are organized in a completely randomized block design with one plant per pot with as factors: two cultivars of leek and four levels of salinity (0, 60, 120, and 240 mM NaCl). The cultivars were well irrigated by fresh saline water for sixty days.
Results:This study demonstrated that increasing salinity reduces the morphological parameters, yield (to 30.43% in Gros long d’été and 30.78% in Monstreux conantan from control to 240 mM NaCl), ascorbic acid, and mineral distribution of the plant for all varieties. We notice that salinity increases osmolytes, antioxidant components, and Na content in the stem and leaves, with a larger accumulation in Gros long d’été than Monstreux conantan. The variety Gros long d’été has proven to be the most tolerant even in the presence of the highest concentration (240 mM NaCl).
Conclusion: Gros long d’été cultivar maintains great productiveness and nutritional quality in high levels of salinity; its salt-tolerance is higher than Monstreux conantan cultivar. The good behaviour of Gros long d’été cultivar facing salinity can be considered for its use to better enhance the Sahelian and coastal areas.
Downloads
Article Details
Copyright (c) 2025 Hand MJ, et al.

This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing and protecting the author rights is the central aim and core of the publishing business. Peertechz dedicates itself in making it easier for people to share and build upon the work of others while maintaining consistency with the rules of copyright. Peertechz licensing terms are formulated to facilitate reuse of the manuscripts published in journals to take maximum advantage of Open Access publication and for the purpose of disseminating knowledge.
We support 'libre' open access, which defines Open Access in true terms as free of charge online access along with usage rights. The usage rights are granted through the use of specific Creative Commons license.
Peertechz accomplice with- [CC BY 4.0]
Explanation
'CC' stands for Creative Commons license. 'BY' symbolizes that users have provided attribution to the creator that the published manuscripts can be used or shared. This license allows for redistribution, commercial and non-commercial, as long as it is passed along unchanged and in whole, with credit to the author.
Please take in notification that Creative Commons user licenses are non-revocable. We recommend authors to check if their funding body requires a specific license.
With this license, the authors are allowed that after publishing with Peertechz, they can share their research by posting a free draft copy of their article to any repository or website.
'CC BY' license observance:
|
License Name |
Permission to read and download |
Permission to display in a repository |
Permission to translate |
Commercial uses of manuscript |
|
CC BY 4.0 |
Yes |
Yes |
Yes |
Yes |
The authors please note that Creative Commons license is focused on making creative works available for discovery and reuse. Creative Commons licenses provide an alternative to standard copyrights, allowing authors to specify ways that their works can be used without having to grant permission for each individual request. Others who want to reserve all of their rights under copyright law should not use CC licenses.
Theunissen J, Schelling G. Infestation of leek by thrips tabaci as related to spatial and temporal patterns of undersowing. Bio Control. 1998;43(1):107-11. Available from: https://research.wur.nl/en/publications/infestation-of-leek-by-thrips-tabaci-thysanoptera-thripidae-as-re/
Koca I, Tasci B. Mineral composition of leek. Acta Hortic. 2016;1143:147-151. Available from: https://doi.org/10.17660/ActaHortic.2016.1143.21
Ben Arfa A, Najjaa H, Yahia B, Tlig A, Neffati M. Antioxidant capacity and phenolic composition as a function of genetic diversity of wild Tunisian leek (Allium ampeloprasum L.). Acad J Biotechnol. 2015;3:15-26. Available from: https://doi.org/10.15413/AJB.2015.0121
Radovanović B, Mladenović J, Radovanović A, Pavlović R, Nikolić V. Phenolic composition, antioxidant, antimicrobial and cytotoxic activities of Allium porrum L. (Serbia) extracts. J Food Nutr Res. 2015;3:564-569. Available from: https://pubs.sciepub.com/jfnr/3/9/1/index.html
Fattorusso E, Lanzotti V, Taglialatela-Scafati O, Cicala C. The flavonoids of leek, Allium porrum. Phytochemistry. 2001;57:565-569. Available from: https://doi.org/10.1016/s0031-9422(01)00039-5
Ahanger MA, Agarwal RM. Salinity stress-induced alterations in antioxidant metabolism and nitrogen assimilation in wheat (Triticum aestivum L) as influenced by potassium supplementation. Plant Physiol Biochem. 2017;115:449-60. Available from: https://doi.org/10.1016/j.plaphy.2017.04.017
Ahmad P, Ahanger MA, Alam P, Alyemeni MN, Wijaya L, Ali S, Ashraf M. Silicon (Si) supplementation alleviates NaCl toxicity in mung bean [Vigna radiata (L.) wilczek] through the modifications of physio-biochemical attributes and key antioxidant enzymes. J Plant Growth Regul. 2019;38(1):70-82. Available from: https://doi.org/10.1007/s00344-018-9810-2
Gupta B, Huang B. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics. 2014;2014:701596. Available from: https://doi.org/10.1155/2014/701596
Beltagi MS. Exogenous ascorbic acid (vitamin C) induced anabolic changes for salt tolerance in chick pea (Cicer arietinum L.) plants. African Journal of Plant Science. 2008;2(10):118-123. Available from: https://www.researchgate.net/publication/228661116_Exogenous_ascorbic_acid_vitamin_C_induced_anabolic_changes_for_salt_tolerance_in_chick_pea_Cicer_arietinum_L_plants
Khan MIR, Asgher M, Khan NA. Alleviation of salt-induced photosynthesis and growth inhibition by salicylic acid involves glycine betaine and ethylene in mungbean (Vigna radiata L.). Plant Physiol Biochem. 2014;80:67–74. Available from: https://doi.org/10.1016/j.plaphy.2014.03.026
Sarker U, Islam MT, Rabbani MG, Oba S. Genetic variation and interrelationship among antioxidant, quality, and agronomic traits in vegetable amaranth. Turkish Journal of Agriculture and Forestry. 2016;40:526–535. Available from: https://doi.org/10.3906/tar-1601-24
Munns R. Comparative physiology of salt and water stress. Plant Cell Environ. 2002;25:239–250. Available from: https://doi.org/10.1046/j.0016-8025.2001.00808.x
Chen H, Jiang JG. Osmotic adjustment and plant adaptation to environmental changes related to drought and salinity. Environ Rev. 2010;18:309–319. Available from: https://cdnsciencepub.com/doi/abs/10.1139/A10-014
Coca CA. NaCl effects on growth, yield, and quality parameters in onion (Allium cepa L.) under controlled conditions. Rev Colomb Cienc Hortic. 2012.
Ayers RS, Westcot. Water quality for agriculture. Irrigation and Drainage Paper No. 29. 1989. Available from: https://www.fao.org/4/t0234e/t0234e00.htm
Munns RA, James A, Lauchli. Approaches to increasing the salt tolerance of wheat and other cereals. J Exp Bot. 2006;57(5):1025‐1043. Available from: https://doi.org/10.1093/jxb/erj100
Imana C, Aguyoh JN, Opiyo A. Growth and physiological changes of tomato as influenced by soil moisture levels. Second RUFORUM Biennial Meeting; Entebbe, Uganda. 2010. Available from: http://repository.ruforum.org/system/tdf/Imana.pdf?file=1&type=node&id=34720&force=
Hoagland DR, Arnon DI. The water culture method for growing plants without soil. University of California, College of Agriculture, Agricultural Experiment Station. 1950. Available from: https://www.scirp.org/reference/referencespapers?referenceid=1850958
Walkley A, Black IA. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 1934;37:29–38. Available from: http://dx.doi.org/10.1097/00010694-193401000-00003
Okalebo JR, Gathua WK, Woomer PL. Laboratory methods of soil and plant analysis: a working manual. Soil Biology and Fertility, Soil Science Society of East Africa, Kari, UNESCO-ROSTA; Nairobi, Kenya. 1993;88. Available from: https://www.scirp.org/reference/referencespapers?referenceid=2505600
Taffouo VD, Djiotie NL, Kenne M, Din N, Priso JR, Dibong S, Akoa A. Effect of salt stress on physiological and agronomic characteristics of three tropical cucurbit species. Journal of Applied Bioscience. 2008;10:434–441. Available from: https://www.m.elewa.org/JABS/2008/10(1)/2.pdf
Taleisnik E, Peyrano G, Arias C. Response of Chloris gayana cultivars to salinity. 1. Germination and early vegetative growth. Trop Grassl. 1997;31:232–240. Available from: https://www.tropicalgrasslands.info/public/journals/4/Historic/Tropical%20Grasslands%20Journal%20archive/PDFs/Vol_31_1997/Vol_31_03_97_pp232_240.pdf
Metwally SA, Khalid KA, Abou-Leila BH. Effect of water regime on the growth, flower yield, essential oil, and proline contents of Calendula officinalis. Nusantara Bioscience. 2013;5(2):65-69. Available from: https://smujo.id/nb/article/view/902
Taffouo VD, Kouamou JK, Ngalangue LMT, Ndjeudji BAN, Amougou A. Effect of salinity stress on growth, ion partitioning and yield of some cowpea (Vigna unguiculata L. Walp.) cultivars. International Journal of Botany. 2009;5(2):135-145. Available from: https://scialert.net/abstract/?doi=ijb.2009.135.143
Sánchez FJ, De Andrés EF, Tenorio JL, Ayerbe L. Growth of epicotyls, turgor maintenance and osmotic adjustment in pea plants (Pisum sativum L.) subjected to water stress. Field Crops Research. 2004;86:81-90. Available from: https://doi.org/10.1016/S0378-4290(03)00121-7
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 1956;28:350-356. Available from: https://pubs.acs.org/doi/10.1021/ac60111a017
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976;72:248-254. Available from: https://doi.org/10.1016/0003-2697(76)90527-3
Bates L, Waldren RP, Teare ID. Rapid determination of free proline for water stress studies. Plant and Soil. 1973;39(2):205-207. Available from: https://link.springer.com/article/10.1007/BF00018060
Van Soest PJ. Use of detergents in the analysis of fibrous feeds. Preparation of fiber residues of low nitrogen content. Journal of the Association of Official Agricultural Chemists. 1963;46:825-829. Available from: https://www.scirp.org/reference/referencespapers?referenceid=1840997
Gossett DR, Millhollon EP, Lucas MC. Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Science. 1994;34:706-714. Available from: https://doi.org/10.2135/cropsci1994.0011183X003400030020x
Gahler S, Otto K, Bohm V. Alterations of vitamin C, total phenolics, and antioxidant capacity as affected by processing tomatoes to different products. Journal of Agricultural and Food Chemistry. 2003;51:7962-7968. Available from: https://doi.org/10.1021/jf034743q
Saeedeh A, Asna U. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chemistry. 2007;102:1233-1240. Available from: https://doi.org/10.1016/j.foodchem.2006.07.013
Ordon JD, Gomez MA, Vattuone MI. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chemistry. 2006;97:452-458. Available from: https://doi.org/10.1016/j.foodchem.2005.05.024
Abreu CA. Comparison of analytical methods for evaluating metal availability in soils. Brazilian Journal of Soil Science. 1995;19:463-468.
Malavolta E, Vitti GC, Oliveira SA. Avaliação do estado nutricional das plantas: princípios e aplicações. 2nd ed. Piracicaba (SP): POTAFOS; 1997;319. Available from: https://www.scirp.org/reference/referencespapers?referenceid=2937675
Pauwels JM, Van Ranst E, Verloo M, Mvondo ZA. Analysis methods of major plant elements. In: Pedology Laboratory Manual: Methods of Plants and Soil Analysis. Publica Agricol; 1992;28. Brussels. Available from: https://www.scirp.org/reference/referencespapers?referenceid=2937676
Ashraf M, Orooj A. Salt stress effects on growth, ion accumulation, and seed oil concentration in an arid zone traditional medicinal plant, ajwain (Trachyspermum ammi [L.] Sprague). J Arid Environ. 2006;64:209–220. Available from: https://doi.org/10.1016/j.jaridenv.2005.04.015
Rebey IB, Bourgou S, Rahali FZ, Msaada K, Ksouri R, Marzouk B. Relation between salt tolerance and biochemical changes in cumin (Cuminum cyminum L.) seeds. J Food Drug Anal. 2017;25:391–402. Available from: https://doi.org/10.1016/j.jfda.2016.10.001
Taârit MB, Msaada K, Hosni K, Marzouk B. Physiological changes, phenolic content and antioxidant activity of Salvia officinalis L. grown under saline conditions. J Sci Food Agric. 2012;92:1614–1619. Available from: https://doi.org/10.1002/jsfa.4746
Kiremit MS, Arslan H. Effects of irrigation water salinity on drainage water salinity, evapotranspiration, and other leek (Allium porrum L.) plant parameters. Scientia Horticulturae. 2016;201:211–217. Available from: https://doi.org/10.1016/j.scienta.2016.02.001
El Jaafari S. Durum wheat breeding for abiotic stress resistance: defining physiological traits and criteria. In: Royo C, Nachit M, Di Fonzo N, Araus JL, editors. Durum wheat improvement in the Mediterranean region: new challenges. Zaragoza: CIHEAM; 2000;251–256. (Options Méditerranéennes: Série A. Séminaires Méditerranéens; 40). Available from: https://om.ciheam.org/om/pdf/a40/00600038.pdf
Karimpour M. Effect of drought stress on RWC and chlorophyll content on wheat (Triticum durum L.) genotypes. World Ess J. 2019;7:52–56.
Ilyas M, Mohammad N, Nadeem K, Ali H, Aamir HK, Kashif H, et al. Drought tolerance strategies in plants: a mechanistic approach. J Plant Growth Regul. 2020. Available from: https://link.springer.com/article/10.1007/s00344-020-10174-5
Passioura JB, Angus JF. Improving productivity of crops in water-limited environments. Adv Agron. 2010;106:37–75. Available from: https://doi.org/10.1016/S0065-2113(10)06002-5
Munns R, Passioura JB, Colmer TD, Byrt CS. Osmotic adjustment and energy limitations to plant growth in saline soil. New Phytol. 2020;225:1096. Available from: https://doi.org/10.1111/nph.15862
Zhao S, Zhang Q, Liu M, Zhou H, Ma C, Wang P. Regulation of plant responses to salt stress. Int J Mol Sci. 2021;22(9):4609. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC8125386/
Bouatrous Y. Water stress correlated with senescence in durum wheat (Triticum durum Desf). Adv Environ Biol. 2013;7(7):1306–1314. Available from: https://www.researchgate.net/publication/259007817_Water_stress_correlated_with_senescence_in_durum_wheat_Triticum_durum_Desf
Hireche R. Response of alfalfa (Medicago sativa L.) to water stress and the depth of the seedlings. Magister Thesis. Univ. EL Hadj Lakhdar, Batna. 2006;83.
Ratnakar A, Rai A. Effect of NaCl salinity on β-carotene, thiamine, riboflavin, and ascorbic acid contents in the leaves of Amaranthus polygamous L. var. Pusa Kirti. Journal of Stress Physiology & Biochemistry. 2013;9:187–192. Available from: https://sciencebeingjournal.com/sites/default/files/Effect%20of%20NaCl%20salinity%20on%20%CE%B2-carotene,%20thiamine,%20riboflavin.pdf
Alam MA, Juraimi AS, Rafii MY, Hamid AA, Aslani F, Alam MZ. Effects of salinity and salinity-induced augmented bioactive compounds in purslane (Portulaca oleracea L.) for possible economic use. Food Chem. 2015;169:439–447. Available from: https://doi.org/10.1016/j.foodchem.2014.08.019
Yarnia M, Benam MBK, Nobari N. The evaluation of grain and oil production, some physiological and morphological traits of amaranth cv. Koniz as influenced by the salt stress in hydroponic conditions. J Agric Food Environ Sci. 2016;69:87–93. Available from: https://agris.fao.org/search/en/providers/122550/records/68b6dbf868d9e6806700a86a
Turkan I, Demiral T. Recent developments in understanding salinity tolerance. Environ Exp Bot. 2009;67:2–9. Available from: https://doi.org/10.1016/j.envexpbot.2009.05.008
Benito B, Haro R, Amtmann A, Cuin TA, Dreyer I. The twins K+ and Na+ in plants. J Plant Physiol. 2014;171:723–731. Available from: https://doi.org/10.1016/j.jplph.2013.10.014
Odjegba VJ, Chukwunwike IC. Physiological responses of Amaranthus hybridus L. under salinity stress. Indian Journal of Innovations and Developments. 2012;1(10):742–748.
Atta K, Pal AK, Jana K. Effect of salinity, drought, and heavy metal stress during the seed germination stage in ricebean (Vigna umbellata (Thunb.) Ohwi and Ohashi). Plant Physiol Rep. 2021;26:109–115. Available from: https://link.springer.com/article/10.1007/s40502-020-00542-4
Shabala S, Pottosin I. Regulation of potassium transport in plants under hostile conditions: implications for abiotic and biotic stress tolerance. Physiologia Plantarum. 2014;151:257–279. Available from: https://doi.org/10.1111/ppl.12165
El Midaoui M, Benbella M, Aït Houssa A, Ibriz M, Talouizte A. Contribution to the study of some salinity adaptation mechanisms in cultivated sunflower (Helianthus annuus L.). Revue THE. 2007;(136):29–34.
Fatma M, Masood A, Per TS, Khan NA. Nitric oxide alleviates salt stress-inhibited photosynthetic performance by interacting with sulfur assimilation in mustard. Front Plant Sci. 2016;25:521. Available from: https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00521/full
Hand MJ, Taffouo VD, Nouck AE, Nyemene KPJ, Tonfack LB, Meguekam TL, Youmbi E. Effects of salt stress on plant growth, nutrient partitioning, chlorophyll content, leaf relative water content, accumulation of osmolytes and antioxidant compounds in pepper (Capsicum annuum L.) cultivars. Not Bot Horti Agrobo. 2017;45(2):481–490. Available from: https://www.notulaebotanicae.ro/index.php/nbha/article/view/10928
Nouck AE, Hand MJ, Numfor EN, Ekwel SS, Ndouma CM, Shang EW, Taffouo VD. Growth, mineral uptake, chlorophyll content, biochemical constituents and non-enzymatic antioxidant compounds of white pepper (Piper nigrum L.) grown under saline conditions. Int J Biol Chem Sci. 2021;15(4):1457–1468.